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KMID : 0861020170320030055
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Korea Journal of Herbology
2017 Volume.32 No. 3 p.55 ~ p.61
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Investigation of the effect of Lithospermi Radix on tight-junction related genes in HaCaT cells
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Cho Nam-Joon
Lee Byeong-Kwon
Lee Woong-Hee
Kim Kee-Kwang
Kim Kyoon-Eon
Han Hyo-Sang
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Abstract
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Objectives : The aim of this research was to determine the diverse effects of Lithospermi Radix Water Extract (LR) on human keratinocyte HaCaT cells, and to examine whether those effects could be applied to the human skin.
Methods : We examined effect of LR on the cell viability of using the MTS assay in human keratinocyte HaCaT cells. The antioxidation effect of LR was analyzed relative to the well-known antioxidant resveratrol, using an ABTS assay. Quantitative RT-PCR analysis revealed that, in HaCaT cells, LR influenced the mRNA expression of tight-junction genes associated with skin moisturization. Furthermore, a wound-healing assay demonstrated altered cell migration in LR-treated HaCaT cells.
Result : The cytotoxicity was confirmed to be higher in LR at a concentration of 800 ㎍/㎖ using the MTS assay in HaCaT cells. In comparison to 100 μM resveratrol, 1,600 ㎍/㎖ LR showed either a similar or superior antioxidation effect. LR treatment in HaCaT cells reduced the mRNA expression levels of claudin 3, claudin 4, claudin 6, claudin 8, and ZO-2 to less than 0.80-fold, whereas JAM-A and Tricellulin mRNA expression level increased more than 1.33- fold. In addition, HaCaT cells migration was decreased to 83.9% by LR treatment.
Conclusions : LR of antioxidation activity will have an anti-aging effect on the skin by reducing oxidative stress. Further studies are required to address the implications for human skin, given LR`s effects of altering mRNA expression of tight junction-related gene and decreasing cell migration of HaCaT cells.
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KEYWORD
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Lithospermi Radix, Cell viability, Anti-oxidant, Tight-junction, Cell migration
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